Cellulose acetate sheets provide a quick and convenient means of replicating surfaces, particularly from large objects which cannot be sectioned

Cellulose acetate film is also used to make replicates of materials and biological samples for microscopy. The techniques were developed for metallographic needs to examine the grain structure of polished metals. Replication can be used to understand the distribution, for example, of different types of iron in carbon steel samples, or the fine distribution of damage to a sample subject to mechanical wear.

For sample preparation, cut out a piece of the replica material of a size suitable to cover the area to be replicated. Place a drop or two of acetone on the specimen surface and apply the replica film immediately. Allow surface tension forces to pull the film down against the specimen; no pressure is required.